枸杞种间杂交F1 群体的SSR鉴定及遗传分析 |
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引用本文:尹 跃,赵建华,何昕孺,梁晓婕,安 巍,秦小雅,曹有龙.枸杞种间杂交F1 群体的SSR鉴定及遗传分析[J].西北农业学报,2019,28(12):2027~2034 |
DOI:10.7606/j.issn.1004-1389.2019.12.015 |
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基金项目:国家自然科学基金地区基金(31760218);宁夏农林科学院科技创新先导资金(NKYJ-17-17);第三批宁夏青年科技人才托举工程(TJGC2018022);全产业链创新示范(QCYL-2018-05)。 |
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中文摘要:以黄果枸杞(Lycium bararum var.auranticarpum ,用P1表示)为父本,北方枸杞(L.chinense var.potaninii,用P2表示)为母本,杂交获得F1 群体91个单株。从66对SSR引物中筛选出具有双亲互补型杂合位点6对引物,对91个F1 单株进行杂种鉴定和遗传变异分析。结果表明:6对引物在91个单株进行扩增检测结果中有83个单株在6个SSR位点上表现为双亲互补型杂合位点,可被认定为真杂种,杂种率为91.2%,另外8个单株出现异常SSR基因型,需要结合细胞学进一步验证。UPGMA聚类分析结果显示:在遗传距离为 0.712处,83个F1 单株被划分为2大类,第Ⅰ大类包括38个F1 单株,占45.8%,第二大类包括45个F1 单株,占54.2%。杂交后代遗传变异大,遗传多样性丰富。 |
中文关键词:枸杞 F1 群体 杂种鉴定 SSR标记 遗传多样性 |
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Hybrid Identification and Genetic Analysis in Wolfberry F1 Population Using SSR Markers |
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Abstract:A total of 91 plants of F1 population were obtained by crossing L.bararum var.auranticarpum as a male parent(expressed in P1) with L.chinense var.potaninii as a female parent(expressed in P2).Six pairs of SSR primers with parental complementary heterozygous loci were screened out from 66 pairs of SSR primers for hybridization identification and genetic variation analysis of those 91 plants.Six pairs of primers were used for amplification experiments.The results indicated that 83 of 91 plants showed parental complementary heterozygous loci on these 6 SSR loci with the heterozygous rate of 91.2%,which could be identified as true hybrids.The other 8 plants showed abnormal SSR genotypes,which needed further verification with cytology.Further UPGMA cluster analysis revealed that the 83 plants of F1 population were divided into two groups at genetic distance of 0.712.The first group included 38 plants(45.8%) and the second group included 45 plants(54.2%).Hybrid progenies have great genetic variation and rich genetic diversity. |
keywords:Wolfberry F1 population Hybrid identification SSR markers Genetic diversity |
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