新疆南疆部分地区 3 种璃眼蜱的分子生物学鉴定 |
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引用本文:李凯瑞 ,李 飞,何 波,张路瑶,赵 丽,刘永宏.新疆南疆部分地区 3 种璃眼蜱的分子生物学鉴定[J].西北农业学报,2018,27(12):1716~1722 |
DOI:10.7606/j.issn.1004-1389.2018.12.002 |
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基金项目:国家自然科学基金(31460655);新疆生产建设兵团塔里木畜牧科技重点实验室开放课题(HS201501,HS201801)。 |
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中文摘要:旨在对新疆南疆部分地区璃眼蜱属的蜱进行分子生物学鉴定,了解其分布情况。采集新疆南疆部分地区蜱,通过形态学鉴定将璃眼蜱属蜱作为样本,通过 12S rDNA和 16S rDNA基因扩增、测序及序列分析进行蜱种类鉴定。13个采样点共559只璃眼蜱属蜱,鉴定为3种,分别为小亚璃眼蜱、亚洲璃眼蜱和残缘璃眼蜱。 |
中文关键词: 12S rDNA 16S rDNA 璃眼蜱属 分子生物学鉴定 |
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Molecular Identification of Three Species of Ticks in Southern Xinjiang |
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Abstract:Molecular identification of Hyalomma ticks in parts of Southern Xinjiang was conducted to understand their distribution. Ticks were collected from parts of Southern Xinjiang and taken as samples of Hyalomma ticks by morphological identification, the species identification was carried out by 12S rDNA and 16S rDNA gene amplification, sequencing and sequence analysis. A total of 559 Hyalomma ticks from 13 sampling sites had been identified as 3 species of Hyalomma ticks, Hyalomma anatolicum, Hyalomma asiaticum, and Hyalomma detritum respectively. The results laid foundation for prevention and control of Hyalomma ticks and tick-borne diseases. |
keywords: 12S rDNA 16S rDNA Hyalomma Molecular identification |
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