| Abstract:The thaumatin-like proteins(TLP) play a role in plant growth, development and stress resistance. The composition, structure, cis-acting element and codon usage bias of TLP family of foxtail millet were analyzed by bioinformatics. The results showed that the foxtail millet TLP family consists of 43 members, distributing on nine chromosomes and being divided into three kinds of gene structure types. Sixteen of 43 members contains merely one exon, and introns phase of 14 members possessing three exons belong to 1-2 type. Phylogenetic analysis classifies into twelve clusters , genes of group five and six are mainly from chromosome Ⅲ and Ⅰ, respectively, and most of the intron phase is 1-2 type. These genes may be originated from the same ancestor, and correlated to the evolution events of chromosome recombination. 88.4% genes are involved in stress responses, and multiple gene promoter regions contain hormones and stress responsive elements, indicating that TLP genes play a role in stress resistance.ENC values of most genes are smaller, the distribution of GC3s value is centralized, and 10 optimal codons ended in G or C are found. It is suggested that codon usage in TLP family of foxtail millet is biased, gene expression of potential is high, and is mainly influenced by natural selection pressure during evolution. |
