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鱼腥草内生真菌多样性分析
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引用本文:崔文霞,陈 睿,张峰华,崔忠华,李勤凡.鱼腥草内生真菌多样性分析[J].西北农业学报,2017,26(10):1513~1519
DOI:10.7606/j.issn.1004-1389.2017.10.014
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作者单位
崔文霞,陈 睿,张峰华,崔忠华,李勤凡 (1.西北农林科技大学 动物医学院陕西杨凌 7121002.动物疾病预防控制中心陕西临潼 710600) 
中文摘要:为探索鱼腥草(Houttuynia cordata Thunb)内生真菌类群组成及多样性,采用组织分离法对不同季节、不同部位的鱼腥草内生真菌进行分离,以形态学和分子生物学方法鉴定,通过分离频率、Shannon Wiener多样性指数等分析鱼腥草内生真菌的多样性。结果表明,分离得到内生真菌3 822 株,分属于19 个属,其中交链孢属Alternaria和镰孢菌属Fusarium为优势菌群,分别达37.55%和18.50%;在夏季分离得到的内生真菌最多;根中分离得到的内生真菌Shannon Wiener多样性指数(H′)最大,为2.06;不同季节中鱼腥草内生真菌春季和夏季的相似性最高,为0.74;不同组织中叶与花的相似性最高,为0.83。鱼腥草内生真菌具有多样性,不同季节和不同组织部位的鱼腥草内生真菌存在差异性。
中文关键词:鱼腥草  内生真菌  优势菌群
 
Diversity of Endophytic Fungi in Houttuynia cordata Thunb
Abstract:The composition and diversity of endophytic fungal taxa in Houttuynia cordata Thunb was analyzed. Endophytic fungi from different seasons (autumn,spring and summer) and different parts (root,stem,flower and leaf) of Houttuynia cordata Thunb were isolated by tissue expand method. Strains were classified by morphology and similarity of internal transcribed spacer (ITS) sequence. Composition,diversity of endophytic fungal community were analyzed by the isolation rate(IR),isolation frequency(IF),Shannon Wiener biodiversity index (H′) and Margalef richness index(R). 3 822 strains from Houttuynia cordata Thunb were divided into 19 genera,55 species,of which Alternaria and Fusarium were predominant,respectively accounting for 37.55% and 18.50% of the total. The endophytic fungi of summer were the most among the 3822 strains. The Shannon Wiener biodiversity index associated with roots was the highest (H′=2.06). The highest Sorensen coefficient was detected in spring and summer(Cs=0.74). Among the different tissues,the highest Sorensen coefficient was in leaf and flower(Cs=0.83).The endophytic fungi in Houttuynia cordata Thunb have abundant diversity. Meanwhile,there were some notable differences between distribution and composition of the endophytic fungi isolated from different seasons and different parts of Houttuynia cordata Thunb.
keywords:Houttuynia cordata Thunb  Endophytic fungi  Dominant population
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