| Abstract:In order to understand the drug resistance phenotype and drug resistance gene of Escherichia coli(E.coli) in the intestinal tract of forest musk deer and its relationship with E.coli in the soil environment of colony house. 66 fresh forest musk deer feces and 20 soil samples were collected from theforest musk deer farms in Maoxian, Dujiangyan, Lixian, Shaanxi, Luding and Hanyuan.By isolation, purification, biochemical test and 16S rRNA gene sequence analysis, 59 strains of enterogenic E.coli of forest musk deer and 12 strains E.coli were isolated from the captive house soil, and its drug sensitivity test was carried out. According to the result of drug sensitivity test, the relevant 12 primer pairs were designed, and the resistance genes of all strains were detected by polymerase chain reaction(PCR). The result indicated that the enterogenic E.coli had strong resistance to penicillin, gentamicin, streptomycin, tetracycline and chloramphenico,the proportion of all strains was 81.36%, 5.08%, 13.56%, 20.34% and 6.78%, respectively. E.coli from the captive house soil showed strong resistance to the same kind of drug except for chloramphenico, and the proportion of all strains was 91.67%, 16.67%, 16.67%, 25.00%, respectively. In enterogenic E.coli, 7 genes’ detection rates were high,while ,in soil E.coli,detection rates of 3 genes’were high. For the same resistance gene, the detection rate of enterogenic E.coli resistance gene was generally higher than that of soil E.coli, for overall results of drug resistance gene detection, there was a consistency between forest musk deer enterogenic E.coli and soil E.coli, which indicated that there may be some interactions. |
