| Abstract:The direct observation method, acetocarmine staining method, I2-KI staining method, inorganic acids treatment method, MTT staining method, TTC staining method and FDA fluorescence detection method wereused to compare and analyzedetection effect in sesame pollen vitality. Thediurnal variations of sesame pollen viability was monitored and the diurnal variation of sesame stigma accessibility was studied by benzidine-hydrogen peroxide method and direct pollination method at different time.The results showed that:(1) direct observation method, TTC staining method and FDA fluorescence detection method were not suitable for detecting sesame pollen viability. The four methods including acetocarmine staining method, I2-KI staining method, inorganic acids treatment method and MTT staining method could be used for detecting sesame pollen viability, among which acetocarmine staining method was asimple and easy way to be observed. (2) the diurnal variation of sesame pollen viability showed a decreasing trend, which was highest from 7:30 to 9:30, and continuously decreased from 11:30 to 17:30, which reached the significant level. (3)the diurnal variation of sesame stigma receptivity measured by benzidine-hydrogen peroxide method showed a trend of risingat first and then falling. The receptivity was high at 7:30 in the morning, then slightly increased, reached the highest value at 9:30, and then it showed a significant decline trend with time delay after 11:30. (4) The results of direct pollination method at different time showed that the later pollination time was, the lower the capsule setting rate was, and the highest capsule setting rate was detected when it was pollinated at 7:30. The relative seed setting rate had high values from 7:30 to 11:30 with no significant difference, and decreased significantly after 13:30. (5) the correlation analysis of four indexes, including pollen viability, stigma receptivity, capsule setting rate and relative seed setting rate, showed significantly positive correlation with each other, and the correlation coefficient between stigma receptivity and relative seed setting rate was the greatest,which reached to 0.940. |
